Purification of the protein X of Streptococcus agalactiae with a monoclonal antibody.
نویسندگان
چکیده
The protein X of Steptococcus agalactiae is a surface antigen included in the typing scheme of group B streptococci (GBS). We have developed a monoclonal antibody to the protein X and used it to purify this antigen by affinity chromatography. Electrophoresis in polyacrylamide, and immunoblotting using the monoclonal antibody or a rabbit antiserum raised with the affinity purified protein X, revealed a major band in the region of 200 kDa and a smaller one at 100 kDa. The isolated protein X will make possible investigations of its potential role in virulence and protection.
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عنوان ژورنال:
- FEMS microbiology letters
دوره 64 2-3 شماره
صفحات -
تاریخ انتشار 1991